Series Vol. 6 , 03 August 2023
* Author to whom correspondence should be addressed.
Fluorescent probe technology was discovered in the 19th century and imaging techniques were applied to microscopy around 1670. Fluorescence microscopic imaging is a classical method for observing the structure of organisms in the life sciences. In this regard, single-molecule fluorescence imaging uses fluorescent probes to label, detect, and analyze individual molecules, helping scientists to clearly observe the activities of individual molecules without disrupting the normal physiological state of the organism. In this paper, the principles and applications of fluorescent probe imaging techniques are described and analyzed. The common detection methods of fluorescent probes are spectrophotometry, electrochemistry, atomic absorption spectroscopy, high performance liquid chromatography, and fluorescence spectroscopy. Rapid detection results can be obtained depending on the specific method.
fluorescent probe technique, imaging, microscopy, fluorescent protein, biology field
1. Zhang, S. H. A review of the principles and applications of biofluorescent probes [J]. Contemporary Chemical Research (02), 36-37 (2019).
2. Chen, Y. C. Design and imaging of novel fluorescent probes for bioinorganic species [D]. Nanjing University (2014).
3. Wang, J. L. Synthesis and application of phenmedazole and rhodamine fluorescent dyes [D]. Hunan University (2014).
4. Lv, Y. Z., Feng, X. Y., Liu, L. X., Zhu, Y. A review on the luminescence mechanism of fluorescent probes [J]. Fujian Analytical Testing 26(02), 25-30 (2017).
5. Wang, C. Y., Li, F. S., Guan, Y. H., Liang, F. K. Theoretical study on the mechanism of hydrogen sulfide detection by fluorescent probe molecules [J]. Journal of Jiangsu Institute of Technology 26(02), 81-86 (2020). DOI:10.19831/j.cnki.2095-7394.2020.02.013.
6. Fang, G., Gao, D. Detection of Cu~(2+) by silicon dioxide nanoparticle fluorescent probes [J]. Journal of Beijing Institute of Petrochemical Technology 18(04), 10-12 (2010).
7. Li, C. L. Fluorescent probe signal amplification strategy and new method of biosensing [D]. Qingdao University of Science and Technology (2020). DOI:10.27264/d.cnki.gqdhc.2020.000244.
8. Hu, G. Design and synthesis of protein sulfhydryl/disulfide bonded fluorescent probes and their biological applications [D]. Lanzhou University (2020). DOI:10.27204/d.cnki.glzhu.2020.003624.
9. Han, J., Burgess, K. Trans-cellular pH fluorescent indicators [J]. Chemical Review 110(5), 2709-2728 (2010).
10. Li, X. H., Gao, X. H., Shi, W., Ma, H. Water-soluble small molecule chromogenic fluorescent probes M. Design strategy [J]. Chemical Review 114(1), 590-659 (2014).
11. Han, J., Loudet, A., Barhoumi, R., Burghardt, R. C., Burgess, K. A ratio pH reporter gene for protein-dye coupling imaging in living cells [J]. Journal of the American Chemical Society 131(5), 1642-1643 (2009).
12. Cui, L. X., Zhang, H. H., Li, M., Zhang, C. H., Zhang, G. M., Shuang, S. M., Dong C. Preparation and spectroscopic study of pH-ratio fluorescent probes [J]. Imaging Science and Photochemistry 36(03), 283-290 (2018).
The datasets used and/or analyzed during the current study will be available from the authors upon reasonable request.