Theoretical and Natural Science

- The Open Access Proceedings Series for Conferences


Theoretical and Natural Science

Vol. 6, 03 August 2023


Open Access | Article

ATAC-seq: A powerful tool for investigating chromatin accessibility and transcription factor binding

Xilin Wang * 1
1 Shenyang Agricultural University

* Author to whom correspondence should be addressed.

Theoretical and Natural Science, Vol. 6, 316-322
Published 03 August 2023. © 2023 The Author(s). Published by EWA Publishing
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Citation Xilin Wang. ATAC-seq: A powerful tool for investigating chromatin accessibility and transcription factor binding. TNS (2023) Vol. 6: 316-322. DOI: 10.54254/2753-8818/6/20230267.

Abstract

Chromatin Transposase Accessibility Sequencing is a new high-throughput sequencing technique developed by Professor William Greenleaf in 2013 which uses DNA transposase to probe chromatin accessibility with Tn5 transposase. This technique, which is simpler and more sensitive than DNase-seq, MNase-seq and FAIRE-seq and requires fewer cells, has been used to study chromatin accessibility using Tn5 transposase. ATAC-seq is important for the study of epigenetic molecular mechanisms because it can map chromatin accessibility on a genome-wide scale, compare open chromatin regions in different tumour samples, compare differences in transcription factor binding between treatments, reveal nucleosome localisation information and transcription factor binding sites, and can be used to locate specific unknown transcription factors, which can be used in combination with other methods to screen for specific transcription factors of interest. It is possible to combine this approach with others to investigate specific regulatory factors. Herein, ATAC-seq is systemically profiled to present that ATAC-seq has enormous potential to drive future discoveries in the field of genomics and beyond.

Keywords

ATAC-seq, Tn5 transposase, chromatin accessibility, epigenetic molecular mechanisms, genome-wide scale

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Data Availability

The datasets used and/or analyzed during the current study will be available from the authors upon reasonable request.

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Volume Title
Proceedings of the International Conference on Modern Medicine and Global Health (ICMMGH 2023)
ISBN (Print)
978-1-915371-65-2
ISBN (Online)
978-1-915371-66-9
Published Date
03 August 2023
Series
Theoretical and Natural Science
ISSN (Print)
2753-8818
ISSN (Online)
2753-8826
DOI
10.54254/2753-8818/6/20230267
Copyright
03 August 2023
Open Access
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

Copyright © 2023 EWA Publishing. Unless Otherwise Stated