Theoretical and Natural Science
- The Open Access Proceedings Series for Conferences
Theoretical and Natural Science
Vol. 4, 28 April 2023
Open
Access | Article
Application and Prospect of CRISPR/Cas9 Technology in Marine Diatoms
* Author to whom correspondence should be addressed.
Abstract
CRISPR/Cas9 technology is a third-generation artificial nuclease technology that has been successfully applied in several fields, given its simplicity and efficiency. Although it has been widely used, its application in marine organisms, especially in eukaryotic marine phytoplankton represented by marine diatoms, is just beginning. As one of the most important marine primary producers, marine diatoms have a variety of irreplaceable ecological significance and potential applications, and therefore need the support of genetic engineering technologies to advance their further development. This paper will first introduce the mechanism of action and the history of CRISPR/Cas9 technology. It will also present the application of the CRISPR/Cas9 system in the genetic engineering of marine diatoms and discuss its prospects.
Keywords
genome editing, marine diatom, CRISPR/Cas9
References
1. Nawaly, H, Tsuji, Y & Matsuda, Y 2020, ‘Rapid and precise genome editing in a marine diatom, Thalassiosira pseudonana by Cas9 nickase (D10A)’, Algal Research, vol. 47, p. 101855.
2. Scherer, S & Davis, RW 1979, ‘Replacement of chromosome segments with altered DNA sequences constructed in vitro.’, Proceedings of the National Academy of Sciences, vol. 76, no. 10, pp. 4951–4955.
3. Marintcheva, B 2018, ‘Viral Tools for Genome Manipulations In Vivo’, Harnessing the Power of Viruses, pp. 69–102.
4. Kim, YG, Cha, J & Chandrasegaran, S 1996, ‘Hybrid restriction enzymes: zinc finger fusions to Fok I cleavage domain.’, Proceedings of the National Academy of Sciences, vol. 93, no. 3, pp. 1156–1160.
5. Urnov, FD, Rebar, EJ, Holmes, MC, Zhang, HS & Gregory, PD 2010, ‘Genome editing with engineered zinc finger nucleases’, Nature Reviews Genetics, vol. 11, no. 9, pp. 636–646.
6. Joung, JK & Sander, JD 2012, ‘TALENs: a widely applicable technology for targeted genome editing’, Nature Reviews Molecular Cell Biology, vol. 14, no. 1, pp. 49–55.
7. Janik, E, Niemcewicz, M, Ceremuga, M, Krzowski, L, Saluk-Bijak, J & Bijak, M 2020, ‘Various Aspects of a Gene Editing System—CRISPR–Cas9’, International Journal of Molecular Sciences, vol. 21, no. 24.
8. Mojica, FJM, Díez-Villaseñor, C, García-Martínez, J & Soria, E 2005, ‘Intervening Sequences of Regularly Spaced Prokaryotic Repeats Derive from Foreign Genetic Elements’, Journal of Molecular Evolution, vol. 60, no. 2, pp. 174–182.
9. Hale, CR, Zhao, P, Olson, S, Duff, MO, Graveley, BR, Wells, L, Terns, RM & Terns, MP 2009, ‘RNA-Guided RNA Cleavage by a CRISPR RNA-Cas Protein Complex’, Cell, vol. 139, no. 5, pp. 945–956.
10. Garneau, JE, Dupuis, M-È, Villion, M, Romero, DA, Barrangou, R, Boyaval, P, Fremaux, C, Horvath, P, Magadán, AH & Moineau, S 2010, ‘The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA’, Nature, vol. 468, no. 7320, pp. 67–71.
11. Hopes, A, Nekrasov, V, Kamoun, S & Mock, T 2016, ‘Editing of the urease gene by CRISPR-Cas in the diatom Thalassiosira pseudonana’, Plant Methods, vol. 12, no. 1.
12. Nymark, M, Sharma, AK, Sparstad, T, Bones, AM & Winge, P 2016, ‘A CRISPR/Cas9 system adapted for gene editing in marine algae’, Scientific Reports, vol. 6, no. 1.
Data Availability
The datasets used and/or analyzed during the current study will be available from the authors upon reasonable request.
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License. Authors who publish this series agree to the following terms:
1. Authors retain copyright and grant the series right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this series.
2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the series's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this series.
3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See Open Access Instruction).
- Volume Title
- Proceedings of the 2nd International Conference on Biological Engineering and Medical Science (ICBioMed 2022), Part II
- ISBN (Print)
- 978-1-915371-27-0
- ISBN (Online)
- 978-1-915371-28-7
- Published Date
- 28 April 2023
- Series
- Theoretical and Natural Science
- ISSN (Print)
- 2753-8818
- ISSN (Online)
- 2753-8826
- DOI
- 10.54254/2753-8818/4/20220502
- Copyright
- 28 April 2023
- Open Access
- This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited