Theoretical and Natural Science
- The Open Access Proceedings Series for Conferences
Theoretical and Natural Science
Vol. 6, 03 August 2023
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Bacteriostasis of bacillus sp. 22T against penicillium expansum and comparison between its ability on pear and grape
* Author to whom correspondence should be addressed.
Abstract
In the biological lab of Zhejiang University, we justified and tested a type of bacillus to improve the anti-bacteria ability of commonly affecting diseases of fruits, such as pear and grape, which would lead to a large amount of economic loss and unpredictable negative influence if they were rotten before being sold. Using techniques such as gram staining and bacteriostasis test, this paper explores and confirms the results that bacillus sp. 22T has a strong ability to inhibit the growth of penicillium expansum on pear and grape. After 96 hours of exposure to fermentation broth containing bacillus sp. 22T to suppress the growth of penicillium expansum, 33.33 percent of pear and 16.66 percent of grape were not impacted by the bacterium.
Keywords
bacillus sp. 22T, penicillium expansum, fruit, bacteriostasis, gram staining
References
1. Zhang, S; Ma, M; Zhang, H; Wang, L; et al. Genome-wide analysis of polygalacturonase gene family from pear genome and identification of the member involved in pear softening. BMC Plant Biol. 2019-12-27;19(1):587.
2. Cao, GT; Zhan, XA; Zhang, LL; Yang, CM; et al. Modulation of broilers' caecal microflora and metabolites in response to a potential probiotic Bacillus amyloliquefaciens. J ANIM PHYSIOL AN N. 2018-04-01;102(2):e909-e917.
3. Matichenkov, V; Bocharnikova, E; Romanova, A; Doullet, P. Growth of Bacillus amyloliquefaciens as influence by Si nutrition. ARCH MICROBIOL. 2021-09-01;203(7):4329-4336.
4. Evrendilek, GA; Tok, FM; Soylu, EM; Soylu, S; Inactivation of Penicillum expansum in sour cherry juice, peach and apricot nectars by pulsed electric fields. FOOD MICROBIOL. 2008-08-01;25(5):662-7.
5. Stöcklein, W; Sztajer, H; Menge, U; Schmid, RD; Purification and properties of a lipase from Penicillium expansum. BIOCHIM BIOPHYS ACTA. 1993-06-12;1168(2):181-9.
6. Zhang, Z; Yang, J; Xu, L; Yan, Y; et al. Cloning, codon optimization and expression of mature lipase gene Penicillium expansum. Wei Sheng Wu Xue Bao. 2010-02-01;50(2):228-35.
7. Liu, T; Zhu, L; Zhang, Z; Huang, H; et al. Draft genome sequence of Bacillus sp. M13(2017), a multidrug-resistant subclass B1 blaNDM-producing, spore-forming bacterium isolated from China. J GLOB ANTIMICROB RE. 2018-09-01;14:152-153.
8. Sivaramaiah, N; Malik, DK; Sindhu, SS; Improvement in symbiotic efficiency of chickpea (Cicer arietinum) by coinoculation of Bacillus strains with Mesorhizobium sp. Cicer. INDIAN J MICROBIOL. 2007-03-01;47(1):51-6.
9. Coico, R; Gram staining. Curr Protoc Microbiol. 2005-10-01;Appendix 3:Appendix 3C.
10. Wouthuyzen-Bakker, M; Shohat, N; Sebillotte, M; Soriano, A; et al. Is Gram staining still useful in prosthetic joint infections? J Bone Jt Infect. 2019-01-29;4(2):56-59.
Data Availability
The datasets used and/or analyzed during the current study will be available from the authors upon reasonable request.
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- Volume Title
- Proceedings of the International Conference on Modern Medicine and Global Health (ICMMGH 2023)
- ISBN (Print)
- 978-1-915371-65-2
- ISBN (Online)
- 978-1-915371-66-9
- Published Date
- 03 August 2023
- Series
- Theoretical and Natural Science
- ISSN (Print)
- 2753-8818
- ISSN (Online)
- 2753-8826
- DOI
- 10.54254/2753-8818/6/20230118
- Copyright
- 03 August 2023
- Open Access
- This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited