Theoretical and Natural Science
- The Open Access Proceedings Series for Conferences
Theoretical and Natural Science
Vol. 6, 03 August 2023
Open
Access | Article
Principles and applications of fluorescent probe imaging technology
* Author to whom correspondence should be addressed.
Abstract
Fluorescent probe technology was discovered in the 19th century and imaging techniques were applied to microscopy around 1670. Fluorescence microscopic imaging is a classical method for observing the structure of organisms in the life sciences. In this regard, single-molecule fluorescence imaging uses fluorescent probes to label, detect, and analyze individual molecules, helping scientists to clearly observe the activities of individual molecules without disrupting the normal physiological state of the organism. In this paper, the principles and applications of fluorescent probe imaging techniques are described and analyzed. The common detection methods of fluorescent probes are spectrophotometry, electrochemistry, atomic absorption spectroscopy, high performance liquid chromatography, and fluorescence spectroscopy. Rapid detection results can be obtained depending on the specific method.
Keywords
fluorescent probe technique, imaging, microscopy, fluorescent protein, biology field
References
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Data Availability
The datasets used and/or analyzed during the current study will be available from the authors upon reasonable request.
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- Volume Title
- Proceedings of the International Conference on Modern Medicine and Global Health (ICMMGH 2023)
- ISBN (Print)
- 978-1-915371-65-2
- ISBN (Online)
- 978-1-915371-66-9
- Published Date
- 03 August 2023
- Series
- Theoretical and Natural Science
- ISSN (Print)
- 2753-8818
- ISSN (Online)
- 2753-8826
- DOI
- 10.54254/2753-8818/6/20230139
- Copyright
- 03 August 2023
- Open Access
- This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited