Theoretical and Natural Science
- The Open Access Proceedings Series for Conferences
Theoretical and Natural Science
Vol. 33, 08 March 2024
Open
Access | Article
Research on the application of CRISPR/Cas9 gene editing technology in traditional Chinese medicine
* Author to whom correspondence should be addressed.
Abstract
Traditional Chinese medicine (TCM) is the collective term for the medical practices of all ethnic groups in China (both Han Chinese and ethnic minorities), reflecting the Chinese understanding of life, health, and illness over thousands of years. In addition, traditional herbal medicine has demonstrated its effectiveness in treating complex diseases that have challenged modern Western medicine, such as the use of Artemisia annua extracts to treat malaria, preparations based on traditional Chinese pharmacology to combat large-scale influenza outbreaks, and herbal preparations as an adjunctive therapy to cancer treatment. Currently, the development of traditional Chinese medicine is facing problems such as the loss of prescription knowledge and the degradation of the quality of cultivated species of herbs. This paper uses a literature review to analyze the application of CRISPR/Cas9 gene editing technology in medicinal plants. It also proposes directions that can solve the challenges facing traditional Chinese medicine based on the arrangement of existing literature, and foresees the future application of CRISPR/Cas9 gene editing technology in medicinal plant editing.
Keywords
CRISPR/Cas9, gene function, Traditional Chinese medicine, disease resistance and stress resistance, molecular breeding
References
1. Wu F..Problems of the Traditional Chinese Medicine Industry and Challenges in the 21st Century[J].Information on Traditional Chinese Medicine, 2003(05):61-62.
2. Ishino Y, Shinagawa H, Makino K, et al. Nucleotide sequence of the iap gene, responsible for alkaline phosphatase isozyme conversion in Escherichia coli, and identification of the gene product [J]. J Bacteriol, 1987,
3. Li J F, Norville J E, Aach J, et al. Multiplex and homologous recombination-mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9 [J]. Nat Biotechnol, 2013, 31(8): 688-691.
4. Xie K B, Yang Y N. RNA-guided genome editing in plants using a CRISPR-Cas system [J]. Mol Plant, 2013, 6(6): 1975-1983.
5. Jinek M, Chylinski K, Fonfara I, et al. A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity [J]. Science, 2012, 337(6096): 816-821.
6. Yin K Q, Gao C X, Qiu J L. Progress and prospects in plant genome editing [J]. Nat Plants, 2017, 3: 17107.
7. Li B, Cui G H, Shen G A, et al. Targeted mutagenesis in the medicinal plant Salvia miltiorrhiza [J]. Sci Rep, 2017, 7: 43320.
8. Hu, B., Application of CRISPR/Cas9 technology to the production of high dansylate salvia trichomes roots[D]. Shandong Agricultural University, 2022.DOI:10.27277/d.cnki.gsdnu.2022.000776.
9. Liu,S. Molecular mechanism of SmJAZ9-SmMYB76 and SmbHLH60 in regulating the biosynthesis of pharmacodynamic components of Salvia miltiorrhiza [D]. Hunan University, 2022.DOI:10.27135/d.cnki.ghudu.2022.000018.
10. Hu, S., Tian, Y., Feng, L., et. al. Functions of the Dendrobium Csl4 gene using CRISPR/Cas9 technology [J]. Molecular Plant Breeding, 2021, 19(8): 2596-2602.
11. Zeng L, Zhang Q , Jiang C, et al. Development of Atropa belladonna L. plants with high-yield hyoscyamine and without its derivatives using the CRISPR/Cas9 system [J]. Int J Mol Sci, 2021, 22(4): 1731.
12. Feng G., Guo, K., Yan, Q., et.al. Theoretical basis of autophagy in the treatment of malignant tumors from qi, blood and fluid theory of Chinese medicine[J]. Journal of New Chinese Medicine, 2019, 51(06): 326-328.
13. Wang, Z., Wang, M., Xiao, H., Study on the role of Chai Hu Saponin D in inducing autophagy in hepatocellular carcinoma cells by regulating mTORC signaling pathway[J]. Chinese Pharmaceutical Journal, 2018, 53(19): 1652-1657.
14. Cheng, H., Duan, Q., Progress on the anti-tumor mechanism of Chaihu Saponin D[J]. Shanxi Journal of Traditional Chinese Medicine, 2021,37(02):57-60.
15. Zhang,B., Huo, C., Li,Y., Effects of Chonglou Saponin Ⅰ on mitochondrial autophagy and the expression of LC3-Ⅱ, LC3-Ⅰ and Caspase-3 in gastric cancer SGC-7901 cells[J]. Guiding Journal of Traditional Chinese Medicine and Pharmacy, 2021, 27(09): 31-35.
16. Luo,Y., Jiang, Y., Li, Y., et.al. Chonglou Saponin ⅠEffect on FOXQ1 and epithelial mesenchymal transition in colon cancer HCT116 cells[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2020, 26(11): 119-123.
17. Gong,Y., Chen, S., Chu, M., et.al. Effects of curcumin on autophagic flow in human gastric cancer AGS cells[J]. Chinese Traditional Patent Medicine, 2018, 40(03): 699-702.
18. Liu, F., Li, G., Li, X., et.al.Picrasidine induces autophagy in esophageal cancer Ec109 cells by inhibiting the PI3K/Akt/mTOR signaling pathway[J]. Journal of Xi’an Jiaotong University (Medical Sciences), 2020, 41(03): 468-472
19. Liu, L., Wang, N., Xu, D,. et.al. Mechanisms of the treatment of neocoronary pneumonia with Chinese medicinal preparations[J]. Chemistry of life, 2022,42(12): 2222-2230.DOI:10.13488/j.smhx.20220105.
Data Availability
The datasets used and/or analyzed during the current study will be available from the authors upon reasonable request.
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- Volume Title
- Proceedings of the 2nd International Conference on Modern Medicine and Global Health
- ISBN (Print)
- 978-1-83558-323-4
- ISBN (Online)
- 978-1-83558-324-1
- Published Date
- 08 March 2024
- Series
- Theoretical and Natural Science
- ISSN (Print)
- 2753-8818
- ISSN (Online)
- 2753-8826
- DOI
- 10.54254/2753-8818/33/20240931
- Copyright
- 08 March 2024
- Open Access
- This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited