Theoretical and Natural Science
- The Open Access Proceedings Series for Conferences
Vol. 20, 20 December 2023
* Author to whom correspondence should be addressed.
The combination of muscle cells and soft robotics technology has led to the rapid development of biohybrid robots in recent years. Muscle-driven systems had advantages such as joint flexibility, self-repair, and multi-signal perception, demonstrating unique advantages as an effective technological solution for robot drive systems. Current research has many shortcomings in material properties and muscle stability in 3D muscle tissue cultured in vitro, making it difficult to successfully apply the technology. In this study, we developed a new type of interpenetrating hydrogel network that, combined with 3D cell culture and tissue-induced culture, enabled 3D muscle tissue culture in a hydrogel environment and induced differentiation in muscle tissue. The results of this research provided a foundation for future studies on in vitro muscle tissue culture and the implementation of robots in this field.
muscle-driven systems, interpenetrating hydrogel network, skeletal muscle tissue, horse serum.
1. Duffy RM, Feinberg AW. Engineered skeletal muscle tissue for soft robotics: fabrication strategies, current applications, and future challenges. Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2014;6(2):178-195. doi:10.1002/wnan.1254
2. Gao L, Akhtar MU, Yang F, et al. Recent progress in engineering functional biohybrid robots actuated by living cells. Acta Biomater. 2021;121:29-40. doi:10.1016/j.actbio.2020.12.002
3. Li Z, Seo Y, Aydin O, et al. Biohybrid valveless pump-bot powered by engineered skeletal muscle. Proc Natl Acad Sci U S A. 2019;116(5):1543-1548. doi:10.1073/pnas.1817682116
4. Dumont NA, Bentzinger CF, Sincennes MC, Rudnicki MA. Satellite Cells and Skeletal Muscle Regeneration. Compr Physiol. 2015;5(3):1027-1059. doi:10.1002/cphy.c140068
5. CVETKOVIC C , RAMAN R , CHAN V , et al. Three-dimensionally printed biological machines powered by skeletal muscle[J]. Proc. Natl. Acad. Sci. U. S. A, 2014, 111(28): 10125-10130
6. Aydin O, Zhang X, Nuethong S, et al. Neuromuscular actuation of biohybrid motile bots. Proc Natl Acad Sci U S A. 2019;116(40):19841-19847. doi:10.1073/pnas.1907051116
7. Cvetkovic C, Raman R, Chan V, et al. Three-dimensionally printed biological machines powered by skeletal muscle. Proc Natl Acad Sci U S A. 2014;111(28):10125-10130. doi:10.1073/pnas.1401577111
8. Ricotti L, Menciassi A. Bio-hybrid muscle cell-based actuators. Biomed Microdevices. 2012;14(6):987-998. doi:10.1007/s10544-012-9697-9.
9. Yuya Morimoto, Hiroaki Onoe, and Shoji Takeuchi, "Biohybrid robot with skeletal muscle tissue covered with a collagen structure for moving in air", APL Bioengineering 4, 026101 (2020) https://doi.org/10.1063/1.5127204
10. Cheng KF, Her WY, Liu TS, Chen SC, Liu KM. Primary culture of mouse myoblasts. Gaoxiong Yi Xue Ke Xue Za Zhi. 1995;11(6):306-314.
11. Jo, B., Morimoto, Y., & Takeuchi, S. (2022). Skeletal muscle-adipose co-cultured tissue fabricated using cell-laden microfibers and a hydrogel sheet. Biotechnology and Bioengineering, 119, 636 - 643. https://doi.org/10.1002/bit.27989
12. Chakraborty S, Hentrich T, Wetzel F, et al. PPARgamma induced fatty acid oxidation and mitochondrial gene expression are increased by dietary fish oil and fenofibrate in skeletal muscle cells. Biochim Biophys Acta. 2015;1850(2):352-358. doi:10.1016/j.bbagen. 2014.11.019
13. Pérez-Schindler J, Summermatter S, Salatino S, et al. The corepressor NCoR1 antagonizes PGC-1α and estrogen-related receptor α in the regulation of skeletal muscle function and oxidative metabolism. Mol Cell Biol. 2012;32(3):491-502. doi:10.1128/MCB.06271-11
14. Chen X, Chen H, Li S, et al. A shear-thinning adhesive hydrogel for endovascular embolization. Sci Adv. 2020;6(36):eabb8096. doi:10.1126/sciadv.abb8096
15. Wang C, Huang Y, Pan Y, et al. Preparation and characterization of gellan gum-glyceryl monooleate-glycerol monostearate nanoparticles loaded with edaravone for potential use in ischemic stroke. Int J Nanomedicine. 2015;10:6185-6198. doi:10.2147/IJN.S90323.
16. Zhang Y, Chen W, Feng B, et al. Human embryonic stem cell-derived exosomes promote pressure ulcer healing in aged mice by rejuvenating senescent endothelial cells. Stem Cell Res Ther. 2021;12(1):50. doi:10.1186/s13287-020-02035-3.
The datasets used and/or analyzed during the current study will be available from the authors upon reasonable request.
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