Theoretical and Natural Science

- The Open Access Proceedings Series for Conferences


Theoretical and Natural Science

Vol. 12, 17 November 2023


Open Access | Article

Seeing cells without a lens: Compact 3D digital lensless holographic microscopy for wide-field imaging

Thomas Zhirui Yang * 1 , Yumin Wu 2
1 University of Toronto schools
2 Qingdao University of Technology

* Author to whom correspondence should be addressed.

Advances in Humanities Research, Vol. 12, 61-72
Published 17 November 2023. © 2023 The Author(s). Published by EWA Publishing
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Citation Thomas Zhirui Yang, Yumin Wu. Seeing cells without a lens: Compact 3D digital lensless holographic microscopy for wide-field imaging. TNS (2023) Vol. 12: 61-72. DOI: 10.54254/2753-8818/12/20230434.

Abstract

Optical microscopy is an essential tool for biomedical discoveries and cell diagnosis at micro- to nano-scales. However, conventional microscopes rely on lenses to record 2-D images of samples, which limits in-depth inspection of large volumes of cells. This research project implements a novel 3-D lensless microscopic imaging system that achieves a wide field of view, high resolution, and an extremely compact, cost-effective design: the Digital Lensless Holographic Microscope (DLHM).A lensless holographic microscope is built with only a light source, a sample, and an imaging chip (with other non-essential supporting structures). The entire setup costs $500 to $600. A series of MATLAB-based algorithms were designed to reconstruct phase information of samples simultaneously from the recorded hologram with built-in high-resolution and phase unwrapping functions. This produces 3-D images of cell samples. The 3-D cell reconstruction of biological samples maintained a comparable resolution with conventional optical microscopes while covering a field of view of 36.2 mm2, which is 20-30 times larger. While most microscopes are extremely time-consuming and require professional expertise, the lensless holographic microscope is portable, low-cost, high-stability, and extremely simple. This makes it accessible for point-of-care testing (POCT) to a broader coverage, including developing regions with limited medical facilities.

Keywords

holographic microscope, lens-free imaging, computational imaging

References

1. Sarkar D, Kang J, Wassie A T, Schroeder M E, Peng Z, Tarr T B, Tang A H, Niederst E D, Young J Z, Su H, Park D, Yin P, Tsai L H, Blanpied T A and Boyden E S 2022 Nature Biomedical Engineering 6 1057–1073 ISSN 2157-846X number: 9 Publisher: Nature Publishing Group URL https://www.nature. com/articles/s41551-022-00912-3

2. Wang J, Han M, Roy A R, Wang H, Möckl L, Zeng L, Moerner W E and Qi L S 2022 Cell Reports Methods 2 100170 ISSN 2667-2375 URL https://www.sciencedirect. com/science/article/ pii/S2667237522000224

3. Carlsson K, Danielsson P E, Lenz R, Liljeborg A, Majlöf L and Åslund N 1985 Optics Letters 10 53–55 ISSN 1539-4794 publisher: Optica Publishing Group URL https://opg.optica. org/ol/abstract.cfm? uri=ol-10-2-53

4. Voronin Y M, Didenko I A and Chentsov Y V 2006 Journal of Optical Technology 73 101–110 publisher: S.I.Vavilov Optical Institute URL https://opg.optica.org/jot/abstract.cfm?uri=jot-73-2-101

5. Yanny K, Antipa N, Liberti W, Dehaeck S, Monakhova K, Liu F L, Shen K, Ng R and Waller L 2020 Light: Science & Applications 9 171 ISSN 2047-7538 number: 1 Publisher: Nature Publishing Group URL https://www.nature.com/articles/s41377-020-00403-7

6. Huang T 1971 Proceedings of the IEEE 59 1335–1346 ISSN 1558-2256 conference Name: Proceedings of the IEEE

7. Greenbaum A, Zhang Y, Feizi A, Chung P L, Luo W, Kandukuri S R and Ozcan A 2014 Science Translational Medicine 6 267ra175 ISSN 1946-6242

8. Zhang Y, Greenbaum A, Luo W and Ozcan A 2015 Virchows Archiv 467 3–7 ISSN 1432-2307 URL https://doi.org/10.1007/s00428-015-1782-z

9. Zhang Y, Lee S Y C, Zhang Y, Furst D, Fitzgerald J and Ozcan A 2016 Scientific Reports 6 28793 ISSN 2045-2322 number: 1 Publisher: Nature Publishing Group URL https://www.nature. com/articles/ srep28793

10. Isikman S O, Sencan I, Mudanyali O, Bishara W, Oztoprak C and Ozcan A 2010 Lab on a chip 10 1109–1112 ISSN 1473-0197 URL https://www.ncbi.nlm.nih.gov/pmc/articles/ PMC2869489/

11. Isikman S O, Bishara W, Zhu H and Ozcan A 2011 Applied Physics Letters 98 161109 ISSN 0003-6951 publisher: American Institute of Physics URL https://aip.scitation.org/doi/ 10.1063/1.3548564

12. Mudanyali O, Oztoprak C, Tseng D, Erlinger A and Ozcan A 2010 Lab on a Chip 10 2419–2423 ISSN 1473-0189 publisher: The Royal Society of Chemistry URL https://pubs.rsc.org/en/content/ articlelanding/2010/lc/c004829a

13. Feizi A, Zhang Y, Greenbaum A, Guziak A, Luong M, Chan R Y L, Berg B, Ozkan H, Luo W, Wu M, Wu Y and Ozcan A 2016 Lab on a Chip 16 4350–4358 ISSN 1473-0189 publisher: The Royal Society of Chemistry URL https://pubs.rsc.org/en/content/articlelanding/2016/lc/ c6lc00976j

14. Su T W, Erlinger A, Tseng D and Ozcan A 2010 Analytical Chemistry 82 8307–8312 ISSN 1520-6882

15. Su T W, Xue L and Ozcan A 2012 Proceedings of the National Academy of Sciences 109 16018–16022 publisher: Proceedings of the National Academy of Sciences URL https://www.pnas.org/doi/10.1073/ pnas.1212506109

16. Su T W, Choi I, Feng J, Huang K, McLeod E and Ozcan A 2013 Scientific Reports 3 1664 ISSN 2045-2322 number: 1 Publisher: Nature Publishing Group URL https://www.nature.com/articles/srep01664

17. Goodman J W 2005 Introduction to Fourier Optics (Roberts and Company Publishers) ISBN 0-9747077-2-4 978-0-9747077-2-3

18. Gerchberg R W 1972 Optik 35 237–246 URL https://api.semanticscholar.org/CorpusID: 55691159

19. Jiang Y, Li H, Pang Y, Ling J, Wang H, Yang Y, Li X, Tian Y and Wang X 2022 Frontiers in Physiology 13 ISSN 1664-042X URL https://www.frontiersin.org/articles/10.3389/fphys. 2022.1040777

20. Madsen A E G, Panah M A, Larsen P E, Nielsen F and Glückstad J 2023 Optics Communications 537 129458 ISSN 0030-4018 URL https://www.sciencedirect.com/science/article/pii/ S0030401823002055

21. Cruz M L, Castro A and Arrizón V 2008 Optics and Photonics for Information Processing II vol 7072 (SPIE) pp 330–339 URL https://www.spiedigitallibrary.org/conference-proceedings-of-spie/ 7072/70721C/Phase-retrieval-in-digital-holographic-microscopy-using-a-Gerchberg-Saxton/ 10.1117/12.793894.full

22. Picazo-Bueno J A, Trindade K, Sanz M and Micó V 2022 Sensors 22 553 ISSN 1424-8220 number: 2 Publisher: Multidisciplinary Digital Publishing Institute URL https://www.mdpi.com/1424-8220/22/2/ 553

23. Firestone L, Cook K, Culp K, Talsania N and Preston Jr K 1991 Cytometry 12 195–206 ISSN 1097-0320_eprint: https://onlinelibrary.wiley.com/doi/pdf/10.1002/cyto.990120302 URL https://onlinelibrary. wiley.com/doi/abs/10.1002/cyto.990120302

24. Groen F C A, Young I T and Ligthart G 1985 Cytometry 6 81–91 ISSN 1097-0320 _eprint:https://onlinelibrary.wiley.com/doi/pdf/10.1002/cyto.990060202 URL https:// onlinelibrary. wiley. com/doi/abs/10.1002/cyto.990060202

25. Sobieranski A C, Inci F, Tekin H C, Yuksekkaya M, Comunello E, Cobra D, von Wangenheim A and Demirci U 2015 Light: Science & Applications 4 e346–e346 ISSN 2047-7538 number: 10 Publisher: Nature Publishing Group URL https://www.nature.com/articles/lsa2015119

26. Tobon-Maya H, Zapata-Valencia S, Zora-Guzmán E, Buitrago-Duque C and Garcia-Sucerquia J 2021 Applied Optics 60 A205–A214 ISSN 2155-3165 publisher: Optica Publishing Group URL https://opg.optica. org/ao/abstract.cfm?uri=ao-60-4-A205

27. Amann S, Witzleben M v and Breuer S 2019 Scientific Reports 9 11260 ISSN 2045-2322 number: 1 Publisher: Nature Publishing Group URL https://www.nature.com/articles/s41598-019-47689-1

Data Availability

The datasets used and/or analyzed during the current study will be available from the authors upon reasonable request.

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License. Authors who publish this series agree to the following terms:

1. Authors retain copyright and grant the series right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this series.

2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the series's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this series.

3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See Open Access Instruction).

Volume Title
Proceedings of the 2023 International Conference on Mathematical Physics and Computational Simulation
ISBN (Print)
978-1-83558-135-3
ISBN (Online)
978-1-83558-136-0
Published Date
17 November 2023
Series
Theoretical and Natural Science
ISSN (Print)
2753-8818
ISSN (Online)
2753-8826
DOI
10.54254/2753-8818/12/20230434
Copyright
© 2023 The Author(s)
Open Access
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

Copyright © 2023 EWA Publishing. Unless Otherwise Stated