Theoretical and Natural Science
- The Open Access Proceedings Series for Conferences
Theoretical and Natural Science
Vol. 33, 08 March 2024
Open
Access | Article
Gene sequencing technologies and their application in cancer research
* Author to whom correspondence should be addressed.
Abstract
The continuous development of gene sequencing technology has made the next-generation sequencing technology and the third-generation sequencing technology gradually applied to scientific research, which has greatly improved the efficiency and accuracy of sequencing and reduced the cost. The incidence of tumors in the human population continues to rise, making tumors gradually become one of the most serious diseases that endanger health. In view of the close relationship between gene mutations and tumorigenesis, the progress of gene sequencing technology has a profound impact on clinical research on tumors. In this thesis, literature analysis was used to introduce several gene sequencing technologies and compare them from multiple perspectives. In addition, the causes of tumorigenesis and the role of sequencing technology in the clinical research of human tumors were investigated. This paper found that the third-generation sequencing technology was more efficient than the next-generation sequencing technology, but it was difficult to be widely used due to its lower accuracy. Sequencing technology is of great help to tumor clinical research.
Keywords
Cancer, Gene-sequencing, Application
References
1. Bowne SJ, Sullivan LS, Koboldt DC, et al. Identification of disease-causing mutations in autosomal dominant retinitis pigmentosa (adRP) using next-generation DNA sequencing [J]. Invest Ophthalmol Vis Sci, 2011, 52(1): 494-503.
2. Grossmann V, Schnittger S, Schindela S, et al. Strategy for robust detection of insertions, deletions, and point mutations in CEBPA, a GC-rich content gene, using 454 next-generation deep-sequencing technology [J]. J Mol Diagn,2011, 13(2): 129-136.
3. Imelfort M, Duran C, Batley J, et al. Discovering genetic polymorphisms in next-generation sequencing data [J]. Plant Biotechnol J, 2009, 7(4): 312-317.
4. Konstantina Athanasopoulou, Michaela A. Boti, Panagiotis G. Adamopoulos, Paraskevi C. Skourou, Andreas Scorilas, et al Review Third-Generation Sequencing: The Spearhead towards the Radical Transformation of Modern Genomics [J]. Life, Vol 12, Iss 30, p 30 (2021).
5. Zheng, G.X.Y.; Lau, B.T.; Schnall-Levin, M.; Jarosz, M.; Bell, J.M.; Hindson, C.M.; Kyriazopoulou-Panagiotopoulou, S.; Masquelier, D.A.; Merrill, L.; Terry, J.M.; et al. Haplotyping germline and cancer genomes with high-throughput linked-read sequencing. Nat. Biotechnol. 2016, 34, 303–311. [CrossRef]
6. Deamer, D.; Akeson, M.; Branton, D. Three decades of nanopore sequencing. Nat. Biotechnol. 2016, 34, 518–524.
7. Goodwin, S.; McPherson, J.D.; McCombie, W.R. Coming of age: Ten years of next-generation sequencing technologies. Nat. Rev. Genet. 2016, 17, 333–351
Data Availability
The datasets used and/or analyzed during the current study will be available from the authors upon reasonable request.
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License. Authors who publish this series agree to the following terms:
1. Authors retain copyright and grant the series right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this series.
2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the series's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this series.
3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See Open Access Instruction).
- Volume Title
- Proceedings of the 2nd International Conference on Modern Medicine and Global Health
- ISBN (Print)
- 978-1-83558-323-4
- ISBN (Online)
- 978-1-83558-324-1
- Published Date
- 08 March 2024
- Series
- Theoretical and Natural Science
- ISSN (Print)
- 2753-8818
- ISSN (Online)
- 2753-8826
- DOI
- 10.54254/2753-8818/33/20240885
- Copyright
- 08 March 2024
- Open Access
- This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited