Theoretical and Natural Science
- The Open Access Proceedings Series for Conferences
Theoretical and Natural Science
Vol. 8, 13 November 2023
Open
Access | Article
Loop-mediated Iso-thermal amplification (LAMP) used for COVID-19 detection
* Author to whom correspondence should be addressed.
Abstract
Loop-mediated isothermal amplification (LAMP) is a novel nucleic acid amplification technique, which can be efficiently amplified at constant temperature by using a Bst DNA polymerase with high chain replacement activity. Compared with traditional nucleic acid amplification technology, this technique has the advantages of high sensitivity, strong specificity, simple operation and low detection cost. In the outbreak of COVID-19 in recent years, LAMP technology has been successfully used to develop rapid domestic COVID-19 nucleic acid detection kits with the above advantages, which can achieve rapid detection of COVID-19 in only 20min, greatly improving the convenience and immediacy of nucleic acid detection. In this paper, the principle and development of LAMP technology were summarized by using databases such as NCBI PubMed, Web of Science, CNKI, etc., focusing on the application of LAMP technology in novel coronavirus detection, and the future development direction of LAMP technology was analyzed by describing the latest LAMP technology.
Keywords
Loop-mediated isothermal amplification (LAMP), Isothermal amplification, COVID 19, nucleic acid testing (NAT)
References
1. Kashir J, Yaqinuddin A. Loop mediated isothermal amplification (LAMP) assays as a rapid diagnostic for COVID-19. Med Hypotheses. 2020; 141:109786.
2. Liu Wang, Jin Jinghao, Chen Xiaoren. Advances in loop-mediated isothermal amplification [J]. Advances in Biotechnology, 2019,11(02):128-135.
3. DaoThi VL, Herbst K, Boerner K, et al. A colorimetric RT-LAMP assay and LAMP-sequencing for detecting SARS-CoV-2 RNA in clinical samples. Sci Transl Med. 2020; 12(556):eabc7075.
4. Amaral C, Antunes W, Moe E, et al. A molecular test based on RT-LAMP for rapid, sensitive and inexpensive colorimetric detection of SARS-CoV-2 in clinical samples. Sci Rep. 2021; 11 (1) : 1-12.
5. Choi G, Moehling TJ, Meagher RJ. Advances in RT-LAMP for COVID-19 testing and diagnosis. Expert Rev Mol Diagn. 2023; (1) : 23 September 28.
6. Sun B, Shen F, McCalla SE, et al. Mechanistic evaluation of the pros and cons of digital RT-LAMP for HIV-1 viral load quantification on a microfluidic device and improved efficiency via a two-step digital protocol. Anal Chem. 2013; 85 (3) : 1540-1546.
7. Wei S, Suryawanshi H, Djandji A, et al. Field-deployable, rapid diagnostic testing of saliva for SARS-CoV-2. Sci Rep. 2021; 11 (1) : 5448.
8. Janikova M, Hodosy J, Boor P, et al. Loop-mediated isothermal amplification for the detection of SARS-CoV-2 in saliva. Microb Biotechnol. 2021; 14 (1) : 307-316.
9. Bender AT, Sullivan BP, Lillis L, et al. Enzymatic and Chemical-Based Methods to Inactivate Endogenous Blood Ribonucleases for Nucleic Acid Diagnostics. J Mol Diagn. 2020; 22 (8) : 1030-1040.
10. Lalli MA, Langmade JS, Chen X, et al. Rapid and extraction-free detection of SARS-CoV-2 from saliva by colorimetric reverse-transcription loop-mediated isothermal amplification. Clin Chem. 2021; 67 (2) : 415-424.
11. Rabe BA, Cepko C. SARS-CoV-2 detection using isothermal amplification and a rapid, inexpensive protocol for sample inactivation and purification. Proc Nat Acad Sci. 2020; 117 (39) : 24450-24458.
12. Bokelmann L, Nickel O, Maricic T, et al. Point-of-care bulk testing for SARS-CoV-2 by combining hybridization capture with improved colorimetric LAMP. Nat Commun. 2021; 12 (1) : 1467.
13. Kondo T, Iwatani Y, Matsuoka K, et al. Antibody-like proteins that capture and neutralize SARS-CoV-2. Sci Adv. 2020; 6(42):eabd3916.
14. Huang WE, Lim B, Hsu CC, et al. RT-LAMP for rapid diagnosis of coronavirus SARS-CoV-2. Microb Biotechnol. 2020; 13 (4) : 950-961.
15. Aldossary AM, Tawfik EA, Altammami MA, et al. Development and Validation of Reverse Transcriptase Loop-Mediated Isothermal Amplification (RT-LAMP) as a Simple and Rapid Diagnostic Tool for SARS-CoV-2 Detection. Diagnostics (Basel). 2022; 12 (9) : 2232.
16. Chow FW, Chan TT, Tam AR, et al. A Rapid, Simple, Inexpensive, and Mobile Colorimetric Assay COVID-19-LAMP for Mass On-Site Screening of COVID-19. Int J Mol Sci. 2020; 21 (15) : 5380.
17. Dao Thi VL, Herbst K, Boerner K, et al. A colorimetric RT-LAMP assay and LAMP-sequencing for detecting SARS-CoV-2 RNA in clinical samples. Sci Transl Med. 2020; 12(556):eabc7075.
18. Sherrill-Mix S, Hwang Y, Roche AM, et al. Detection of SARS-CoV-2 RNA using RT-LAMP and molecular beacons. Genome Biol. 2021; 22 (1) : 169.
19. Zhang Y, Chen M, Liu C, et al. Sensitive and rapid on-site detection of SARS-CoV-2 using a gold nanoparticle-based high-throughput platform coupled with CRISPR/Cas12-assisted RT-LAMP. Sens Actuators B Chem. 2021; 345:130411.
20. Nandi SS, Lambe UP, Sawant SA, et al. Development of a RT-LAMP assay for detection of SARS-CoV-2. Indian J Med Res. 2022; 155 (1) : 148-155.
21. Teng F, Cui T, Feng G, et al. Repurposing CRISPR-Cas12b for mammalian genome engineering. Cell Discov. 2018; 3.
22. Joung J, Ladha A, Saito M, et al. Point-of-care testing for COVID-19 using SHERLOCK diagnostics. Preprint. medRxiv. 2020; 2020.05.04.20091231.
23. Ali Z, Aman R, Mahas A, et al. iSCAN: An RT-LAMP-coupled CRISPR-Cas12 module for rapid, sensitive detection of SARS-CoV-2. Virus Res. 2020; 288:198129.
24. Broughton JP, Deng X, Yu G, et al. CRISPR-Cas12-based detection of SARS-CoV-2. Nat Biotechnol. 2020; 38 (7) : 870-874.
25. Zhang Y, Chen M, Liu C, et al. Sensitive and rapid on-site detection of SARS-CoV-2 using a gold nanoparticle-based high-throughput platform coupled with CRISPR/Cas12-assisted RT-LAMP. Sens Actuators B Chem. 2021; 345:130411.
26. Chandrasekaran SS, Agrawal S, Fanton A, et al. Rapid detection of SARS-CoV-2 RNA in saliva via Cas13. Nat Biomed Eng. 2022; 6 (8) : 944-956.
27. Verma MK, Roychowdhury S, Sahu BD, et al. CRISPR-based point-of-care diagnostics incorporating Cas9, Cas12, and Cas13 enzymes advanced for SARS-CoV-2 detection. J Biochem Mol Toxicol. 2022; 36(8):e23113.
28. Huang Hai-Long, Zhu Peng, Yang Hao. LAMP-LFD technology and its application in bioassay [J]. Chinese Journal of Bioengineering,2015,35(12):89-95.
29. Chen X, Zhou Q, Li S, et al. Rapid and Visual Detection of SARS-CoV-2 Using Multiplex Reverse Transcription Loop-Mediated Isothermal Amplification Linked With Gold Nanoparticle-Based Lateral Flow Biosensor. Front Cell Infect Microbiol. 2021; 11:58. 1239.
30. Park BH, Oh SJ, Jung JH, et al. An integrated rotary microfluidic system with DNA extraction, loop-mediated isothermal amplification, and lateral flow strip based detection for point-of-care pathogen diagnostics. Biosens Bioelectron. 2017; 91:334-340.
31. Fang X, Liu Y, Kong J, et al. Loop-mediated isothermal amplification integrated on microfluidic chips for point-of-care quantitative detection of pathogens. Anal Chem. 2010; 82 (7) : 3002-6.
32. Torezin Mendonca G, Cassaboni Stracke M, de Oliveira Coelho B, et al. A new RT-LAMP-on-a-Chip Instrument for SARS-CoV-2 diagnostics. Microchem J. 2022; 180:107600.
33. Colbert AJ, Lee DH, Clayton KN, et al. PD-LAMP smartphone detection of SARS-CoV-2 on chip. Anal Chim Acta. 2022; 1203:339702.
34. Song M, Hong S, Lee LP. Multiplexed Ultrasensitive Sample-to-Answer RT-LAMP Chip for the Identification of SARS-CoV-2 and Influenza Viruses. Adv Mater. 2023; 35(10):e2207138.
35. Rodriguez-Mateos P, Ngamsom B, Walter C, et al. A lab-on-a-chip platform for integrated extraction and detection of SARS-CoV-2 RNA in resource-limited settings. Anal Chim Acta. 2021; 1177:338758.
Data Availability
The datasets used and/or analyzed during the current study will be available from the authors upon reasonable request.
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- Volume Title
- Proceedings of the 2nd International Conference on Modern Medicine and Global Health
- ISBN (Print)
- 978-1-83558-111-7
- ISBN (Online)
- 978-1-83558-112-4
- Published Date
- 13 November 2023
- Series
- Theoretical and Natural Science
- ISSN (Print)
- 2753-8818
- ISSN (Online)
- 2753-8826
- DOI
- 10.54254/2753-8818/8/20240365
- Copyright
- 13 November 2023
- Open Access
- This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited